Cover Image

In vitro litholytic activity of extracts and phenolic fractions of some medicinal plants on urinary stones

Kenza Ammor, Fatima Mahjoubi, Dalila Bousta, Radouane Elhabbani, Abdellah Chaqroune

Abstract


Objective: This study was carried out to evaluate the efficiency of plant extracts used in traditional medicine on the dissolution of oxalo-calcic and cystine stones. Also, the efficiency of phenolic fractions (Tannins and Flavonoids) for the plants that gave us the best stone dissolution rate.

Subjects and methods: Kidney stone of oxalo-calcic and cystine stones were incubated in vitro for 8 weeks in the presence of five plants extracts (hydro-ethanolic and aqueous extracts). NaCl solution (0,9 %) used as negative control and sodium citrate solution at 3 mM/L as a positive control. The studied plants were Herniaria hirsuta L. aerial parts, Opuntia ficus-indica L. flowers, Zea mays L. stigmata, Ammi visnaga L. seeds and Ziziphus lotus L. fruits. After 2, 4, 6 and 8 weeks the stones were weighed after 18 h drying at 40°C and the dissolution rate of the stones and the pH of the solution were measured.

Results: After eight weeks, all the plant extracts and phenolic fractions had revealed a significant effect to dissolve oxalo-calcic and cystine stones in comparison to the control solutions. The best result of cystine stones was showed with aqueous extract of H. hirsuta aerial parts which had a dissolution rate of 88,91 %. Concerning the dissolution rate of oxalo-calcic stones the aqueous extract of Z. mays had the best results with 68 % against 19 % for the sodium citrate solution.  The dissolution rate of the fractions studied showed a better result for Z. mays flavonoids fraction toward oxalo-calcic stones, and Z. mays tannins fraction for cystine stones. The pH undergoes a non-negligible linear increase over the eight weeks for all extracts and phenolic fractions and both kidney stones.

Conclusion: The tested plants' extracts and the phenolic fractions for the plants that gave us the best stone dissolution rate were able to dissolve oxalo-calcic and cystine stones. To confirm the efficiency of these plant extracts on the treatment of oxalo-calcic and cystine stones multiple in vivo tests could be made.


Full Text:

PDF

References


- M. Daudon, RJ Reveillaud, Whewillite and weddellite: toward a different etiopathogenesis the significance of morphological typing of calculi, Nephrol. 1984, 5, 195-201.

- A. Laroubi, M. Touhami, L. Farouk, IRA Zrara, A. Benharref, A. Chait, Prophylaxis effect of Trigonella foenum graecum L. seeds on renal stone formation in rats, Phytother. Res, 2007, 21, 921-925.

- F. Assadi, M. Moghtaderi, Preventive kidney stones. Continue medical education, Int J Prev Med. 2017, 8, 67.

- M. Daudon, CA. Bader, P. Jungers, Urinary calculi: review of classification methods and correlations with etiology, Scanning Microsc. 1993, 7,1081-106.

- S. Bouanani, C. Henchiri, E. Migianu-Griffoni, et al., Pharmacological and toxicological effects of Paronychia argentea in experimental calcium oxalate nephrolithiasis in rats, J Ethnopharmacol. 2010, 129, 38-45.

- F. Atmani, Y. Slimani, AN. Mbark, M. Bnouham, A. Ramdani, in vitro and in vivo anti-lithiasic effect of saponin rich fraction isolated from Herniaria hirsute, J Bras Nefrol, 2006, 28, 199-203.

- A. Khouchlaa, A. Talbaoui, AEY. El idrissi, A. Bouyahya, S. Ait lahsen, A. Kahouadji, M. Tijane, Determination of phenol content and evaluation of in vitro litholytic effects on urolithiasis of moroccan zizyphus lotus L. extract, Phytothérapie, 2018, 16, 14-9.

- F. Meiouet, S. El Kabbaj, M. Daudon, In vitro study of the litholytic effects of herbal extracts on cystine urinary calculi, Prog Urol., 2011, 21, 40-7.

- P. Jungers, D. Joly, MF. Gagnadoux, M. Daudon, cystine lithiasis: physiopathology and medical treatment, Prog urol., 2001, 11, 122-126.

-P. Jungers, M. Daudon, P. Conort, (b) cystine lithiasis. In: renal lithiasis, diagnosis and treatment, Paris Flammarion Médecine Sciences, 1999, 149-161.

-R. Selvam, P. Kalaiselvi, A. Govindaraj, V. Bala Murugan, AS. Sathish Kumar, Effect of A. lanata leaf extract and vediuppu chunnam on the urinary risk factors of calcium oxalate urolithiasis during experimental hyperoxaluria, Pharmacol Res., 2001, 43, 89-93.

-Lipkin M, Shah O, The use of alpha-blockers for the treatment of nephrolithiasis, Rev Urol., 2006, 8, 35-42.

- L. Yachi et al., In vitro litholytic activity of some medicinal plants, African Journal of Urology, 2018, 24, 197-201.

- A. Amar, D. Harrache, F. Atmani, G. Bassou, F. Grillon. Effect of Parietaria officinalis on the crystallization of calcium oxalate in urine, Phytothérapie, 2010, 8, 342-347.

- B. Hannache, D. Bazin, A. Boutefnouchet, M. grass, Effect of medicinal plant extracts on the dissolution of cystine kidney stones in vitro: a mesoscopic study, Progres en urologie, 2012, 22, 577-582.

-J. Bellakhdar, Plantes médicinales au Maghreb et soins de base précis de phytothérapie moderne. Ed le Fennec, 2006, 13-23.

- CA. Newal, LA. Anderson, A Guide for Health-care Professionals, Herbal Medicine, 1996, London.

-F. Grases, J.G. March, M. Ramis, and A. Costa-Bauza, The influence of Zea mays on urinary risk factors for kidney stones in rats, Phytotherapy Research, 1993, 7, 146-149.

-R. Bencheraiet, H. Kherrab, A. Kabouche, Z. Kabouche and M. Jay, Flavonols and antioxidant activity of Ammi visnaga L. (Apiaceae), Rec Nat Prod, 2011, 5, 52-55.

-AO. Abdel-Zaher, SY. Salim, MH. Assaf et RH. Abdel-Hady, Antidiabetic activity and toxicity of Zizyphus spina-christi leaves, J. Ethnopharmacol, 2005, 101,129-138.

-S. Suksamrarn, N. Suwannapoch, N. Aunchai, M. Kuno, P. Ratananukul, R. Haritakum, C. Jansakul et S. Ruchirawat, Ziziphine N, O, P, new antiplasmodial cyclopeptides alkaloids from Zizyphus oenoplia var, brunoniana.Tetrahedron, 2005, 61, 1175-1180.

-L. Benramdane, M. Bouatia, MOB. Idrissi, M. Draoui, Infrared analysisof urinary stones, using a single reflection accessory and KBr pallettransmission, Spectrosc Lett., 2008, 41,72-80.

-L. Estepa, M. Daudon, Contribution of Fourier transform infrared spectroscopy to the identification of urinary stones and kidney crystal deposits, Biospectroscopy 1998, 3, 347-69.

-R. El Habbani, A. Chaqroune, TS. Houssaini, M. Arrayhani, A. Lahrichi, Contribution to the study of the mass reduction of stones by some medicinal plants, Int J Innov Res Sci Eng Technol, 2015, 04(04), 1867–75.

-TE. Grønhaug, S. Glaeserud, M. Skogsrud, et al., Ethno- pharmacological survey of six medicinal plants from Mali, West-Africa, J Ethnobiol Ethnomed, 2008, 4, 26.

-DJ. Simbo, An ethnobotanical survey of medicinal plants in Babungo, Northwest Region, Cameroon, J Ethnobiol Eth- nomed, 2010, 6, 8.

-S. Jennan, R. Fouad, A. Nordine, A. Farah, B. Bennani, S. Moja, H. Greche & F. Mahjoubi, Chemical Composition and Antibacterial Screening of Aerial Parts of Essential Oils of Three Satureja species (Satureja briquetti, Satureja atlantica and Satureja alpina) Growing Wild in the Middle Atlas Mountains of Morocco, Journal of Essential Oil Bearing Plants, 2018, 21(3), 741-748.

- K. Ammor, D. Bousta, S. Jennan, FE. Amarti, R. Lagzizir A. Chaqroune and F. Mahjoubi, Study of Antioxidant, Anti-inflammatory, anti-antinociceptive activities and toxicity of stigmata of Zea mays extracts from Morocco, Phytothérapie, 2019, 10.3166/phyto-2019-0209.

- K. Ammor, D. Bousta, S. Jennan, B. Bennani, A. Chaqroune, and F. Mahjoubi, Phytochemical Screening, Polyphenols Content, Antioxidant Power, and Antibacterial Activity of Herniaria hirsuta from Morocco, The Scientific World Journal, 2018, 7.

- K. Ammor, B. Dalila, J. Sanae, A. Chaqroune, F. Mahjoubi Fatima, Total Polyphenol Content and Antioxidant Power of Ammi visnaga from Morocco, Der Pharma Chemica, 2017, 9, 73-78.

-J. Bruneton, Pharmacognosy, phytochemistry, medicinal plants, Tec et doc édition Lavoisier, 1999, 1120.

-L. Saso, G. Valentini, MG. Leone, E. Grippa, B. Silvestrini, Development of an in vitro assay for the screening of substances capable of dissolving calcium oxalate crystals, Urol Int, 1998, 61, 210-214.

-P. Jungers, Cystine lithiasis, In: Dore B, editor, Les lithiases rénales. Springer-Verlag, 2004, 137-44.

-M. Daudon, Morphoconstitutional analysis of stones in the etiological diagnosis of urinary lithiasis in children, Arch PCdiatr, 2000, 7, 855-65.




DOI: http://dx.doi.org/10.13171/mjc9602001101135ka

Copyright (c) 2020 Mediterranean Journal of Chemistry