Cover Image

Chemical composition and insecticidal activity of Anacyclus pyrethrum essential oil from the Bensliman area against Culex pipiens

Kawtar El Mokhtari, M'hammed EL Kouali, Mohammed Talbi, Latifa Hajji, Abdelhak El Brouzi

Abstract


Anacyclus pyrethrum is a herbaceous plant that belongs to the Asteraceae family. The focus of the present study is to extract the essential oil from this plant, to determine its chemical composition and to evaluate its insecticidal activity against the larvae of the mosquito Culex pipiens. The essential oil from A. pyrethrum collected from the region of Bensliman in Morocco was extracted by hydrodistillation and analyzed by gas chromatography coupled to mass spectrometry were studied. Essential oil yield obtained by hydrodistillation was 0.09%. The major aroma constituents were Spathulenol (20.47%), Germacrene D (16.48%), Caryophyllene oxide (13.20%), 4(14)-Salviale-1-one (8.27%) and Caryophyllene 4(14),8(15)-dien-5α-ol (7.30%). The larvicidal test carried out according to a methodology based on the standard protocol of the World Health Organization was studied on 4th instar larvae of C. pipiens and showed that A. pyrethrum essential oil possesses remarkable insecticidal properties.  After 24 hours of exposition, larvicidal assays revealed a 100% mortality of C. pipiens larvae. The dose of 40 μL/mL was toxic enough to cause 100% larval mortality of C. pipiens. The lethal concentrations LC50 and LC90 calculated for the essential oil studied were of the order of 14.79 μL/mL and 19.95 μL/mL, respectively. To control mosquitoes, this essential oil extracted from A. pyrethrum might be used as a natural insecticide and therefore could be an alternative to synthetic insecticides already present on the market.


Full Text:

PDF

References


- C. Selles, M. Diba, N. Djaboua, F. Beddoub, A. Musellic, B. Tabtia, G. Costac, B. Hammouti, Antimicrobial activity and evolution of the composition of essential oil from Algerian Anacyclus pyrethrum L. through the vegetative cycle, Nat Prod Res., 2013, 27(23), 2231-2234.

- A. Daoudi, L. Nassiri, J. Ibijbijen, A. Boukil, Ethnobotanical study of pyrethrum « Anacyclus pyrethrum L. » in Meknès, El Hajeb, Khénifra Azrou and Ifrane (Morocco), ScienceLib., 2015, 6(140504), 1-27.

- H. Elazzouzi, S. Khennouchi, A. Bentayeb, F. Elhilali, T. Zair, Biocidal effects of alkaloids extracted from Anacyclus pyrethrum L. (Asteraceae) on Callosobruchus maculatus (Fab.) (Coleoptera: Bruchidae), J Innovation and Applied Studies, 2015, 13(4), 756-774.

- I.K. Cvetkovikj, N. Cheliku, G. Stefkov, N. Karapandzova, N. Bardhi, B. Qjazimi, S. Kulevanova, Essential oil composition of five basil cultivars (Ocimum basilicum) from Albania, Mac Pharm Bull., 2015, 61(2),11 – 18.

- A.S. Ilic, M.P. Antic, S.C. Jelacic, T.M. Solevic, Chemical composition of the essential oils of three Ocimum basilicum L. cultivars from Serbia, Not Bot Horti Agrobo, 2019,47(2), 347-351.

- M. Ozcan, C.J. Clause, Essential oil composition of Ocimum basilicum L. and Ocimum minimum L. in Turkey, J Food Sci., 2002, 20(6), 223–228.

- C. Dabire, H.C. Roger, A.B. Nebie, M. Nacro , S. Faustin, Effet du séchage de la matière végétale sur la composition chimique de l’huile essentielle et l’activité antioxydante d’extraits de Ocimum basilicum L, Int J Biol Chem Sci., 2011, 5(3), 1082-1095.

- T. Taechowisan, J. Jantiya, N. Mungchukeatsakul, S. Waya,

P. Phutdhawong, Major Compounds from Ocimum basilicum L. and their antimicrobial activity against methicillin-resistant Staphylococcus aureus, J Scientific Technical Research, 2018, 3(3), 3315-3323.

- D. Benedec, I. Oniga, R. Oprean, M. Tamas, Chemical composition of the essential oils of ocimum basilicum L. cultivated in Romania,

Farmacia, 2008, 57(5), 625-629.

- A. Yahya Al-Maskria, M. Asif Hanifa, M. Yahya Al-Maskarib, A. Susan Abrahama, J. Nasser Al-sabahic, O. Al-Mantheria, Essential Oil from Ocimum basilicum (Omani Basil): A Desert Crop, Nat Prod Commun., 2011, 6(10), 1487-1490.

- S. Ngom, F. Dieng, M. Diop, Composition chimique et propriétés physico-chimiques des huiles essentielles d’Ocimum basilicum et d’Hyptis suaveolens (L.) Poit. Récoltés dans la région de Dakar au Sénégal, Bull Soc R Sci Liège., 2012, 81, 166-175.

- S. Joo Lee, K. Umano, T. Shibamoto, K. Geun Lee, Identification of volatile components in basil (Ocimum basilicum L.) and thyme leaves (Thymus vulgaris L.) and their antioxidant properties, Food Chem., 2004, 91(1), 131–137.

- M. Loredana Soran, S. Codruta Cobzac, C. Varodi, I. Lung, E. Surducan, V. Surducan, The extraction and chromatographic determination of the essentials oils from Ocimum basilicum L by different techniques, Physics, 2009, 182, 12-16.

- H.O. Elansary, K. Yessoufou, S. Shokralla, K. Mahmoud, S. Skalicka, Enhancing mint and basil oil composition and antibacterial activity using seaweed extracts, Ind Crop Prod., 2016, 92, 50–56.

- A. El Ouali Lalami, F. EL-Akhal, R. Guemmouh, H. Greche, Valorization as a bio-insecticide of essential oils of Citrus sinensis and Citrus aurantium cultivated in center of Morocco, J. Mater Environ Sci., 2014, 5 (S1), 2319-2324.

- B. Aouinty, S. Oufara, F. Mellouki, S. Mahari, Évaluation préliminaire de l’activité larvicide des extraits aqueux des feuilles du ricin (Ricinus communis L.) et du bois de thuya (Tetraclinis articulata (Vahl) Mast.) sur les larves de quatre moustiques culicidés : Culex pipiens (Linné), Aedes caspius (Pallas), Culiseta longiareolata (Aitken) et Anopheles maculipennis (Meigen), J. Biotechnol Agron Soc Environ., 2006, 10(2),

-71.

- A. El Ouali Lalami, F. El-Akhal, H. Greche, F. Ouazzani, R. Chahdi, A. Guemmouh, Chemical composition and larvicidal activity of Culex pipiens essential oil of Thymus vulgaris grown in Morocco, J Mater Environ Sci., 2015, 6(1), 214-219.

- P.A. Ntonga, P. Belong, E.M. Bakwo, G.A. Dadji, J.L. Tamesse, Chemical composition and residue activities of Ocimum canum sims and Ocimum basilicum L essential oils on adult female Anopheles funestus ss, J Anim Plant Sci., 2013,19(1), 2854-2863.

- A. Ndomo, A.L. Tapondjou, F. Tendonkeng, F.M. Tchouanguep, Evaluation des propriétés insecticides des feuilles de Callistemon viminalis (Myrtaceae) contre les adultes d’Acanthoscelides obtectus (Say) (Coleoptera; Bruchidae), Tropicultura, 2009, 27(3), 137-143.

- H. Elazzouzi, A. Soro, F. Elhilali, A. Bentayeb, M. Alaoui, M. El Belghiti, T. Zair, Phytochemical study of Anacyclus pyrethrum (L.) of Middle Atlas (Morocco) and in vitro study of antibacterial activity of pyrethrum, Adv in Nat Appl Sci., 2014, 8(8), 31-140.

- M. Aberchane, M. Fechtal, A. Chaouch, T. Bouayoune, Influence of the duration and the extraction technique on the yield and quality of the essential oils of Atlas cedar (Cedrusatlantica manetti), Ann For Res., 2010, 34, 110-118.

- M. Bourkhiss, M. Ouhssine, A. Chaouch, B. Satrani, Effet de séchage sur la teneur et la composition chimique des huiles essentielles de Tetraclinis articulata (Vahl) Masters, Agrosolutions, 2009, 20(1), 1-4.

- O.O. Okoh, A. Sadimenko, A.J. An, The Effects of Age on the Yield and Composition of the Essential Oils of Calendula officinalis, J Applied Sci., 2007, 7, 3806-3810.

- M. Bourkhiss, M. Hnach, T. Lakhlifi, A. Boughdad, A. Farah, B. Satrani, Effect of Age and Vegetative Stage on the Content and Chemical Composition of Essential Oils of Thuja Articulata, Technologies de Laboratoire, 2011, 6(23), 64-68.

- S. Al Ahl, H.A.H. Hasnaa, S.A. Hendawy, Effect of potassium humate and nitrogen fertilizer on herb and essential oil of oregano under different irrigation intervals, J Appl Sci., 2009, 2, 319-323.

- S.F. Hendawy, K.A. Khalid, Effect of chemical and organic fertilizers on yield and essential oil of chamomile flower heads, Med Aromat Plant Sci Biotechnol, 2011, 5, 43-48.

- T. Zair, S. Khannouchi, F. Elhilali, Biocidal effects of aqueous extract of the roots of Anacyclus pyrethrum (Asteraceae) on Callosobruchus maculatus (Fab.) (Coleoptera: Bruchidae), Mediterr. J. Chem., 2012, 1(6),

-325.

- F. Remok, Y. Ait Aicha, S. Khannouchi, T. Zair, Phytochemical and biological studies of Anacyclus pyrethrum L, International Colloquium on Valorization of natural and synthetic biomolecules in Novel drug-vector nanostructures, 15, 16 April 2019, p52.

- G. Aćimović, V. Tešević, M. Todosijević, I. Oljača, K. Dolijanović, Essential oil content and composition of aniseed, J Nat Sci Res., 2015, (128), 67-75.

- A. Orava, A. Raalb, E. Arakb, M. Müüriseppa, T. Kailasa, Composition of the essential oil of Artemisia absinthium L. of different geographical origin, J Proc Estonian Acad Sci Chem., 2006, 55(3), 155–165.

- A. Mambrí, J. Andriolo, M. Manfron, S. Pinheiro, F. Cardoso, M. Neves, Yield and composition of lavender essential oil grown in substrate, Hortic Bras, 2018, 36(2), 259-264.

- K. Norajit, N. Laohakunjit, O. Kerdchoechuen, Antibacterial effect of five Zingiberaceae essential oils, Molecules, 2007, 12(8), 2047-2060.

- H. Baydar, O. Sagdic, G. Ozkan, T. Karadogan, Antibacterial activity and composition of essential oils from Origanum, Thymbra and Satureja species with commercial importance in Turkey, Food Control, 2004, 15(3), 169-172.

- H. Fodil, M. Sarri, N. Hendel, F. Maggi, D. Sarri, Essential oil composition of aerial parts from Algerian Anacyclus monanthos subsp. cyrtolepidioides (pomel) humphries, Nat Prod Res., 2019, 33(2), 292-295.

- A.R. Dashipour, M. Reza, Shahraki, J. Dehvari, M. Shahrakipoor, E. Shahreki, A. Sharaki, The effects of Anacyclus pyrethrum alcoholic root extract on FSH, LH, testosterone and sperm count in diabetic male rats, J Med Sci., 2019, 21(2).

- R. Pavela, P. Sedlák, Post-application temperature as a factor influencing the insecticidal activity of essential oil from Thymus vulgaris, Ind Crops Prod., 2018, 113, 46-49.

- R. Pavela, Acute, synergistic and antagonistic effects of some aromatic compounds on the Spodoptera littoralis Boisd. (Lep, Noctuidae) larvae, Ind Crops Prod., 2014, 60, 247-258.

- D. Annik Boussou, S. Mangelinckx, H. Yedmonhan, P. Boko, M. Akogbeto, N.D. Kimpe, F. Avlessi, D.C.K. Sohounhloue, Chemical composition and insecticidal activity of plant essential oils from Benin against Anopheles gambiae (Giles), Parasit Vectors, 2013, 6(337). https://doi.org/10.1186/1756-3305-6-337.

- A. Urzúa, R. Santander, J. Echeverria, N. Cabezas, S.M. Palacios, Y. Rossi, Insecticide Properties of the Essential Oils from Haplopappus foliosus and Bahia ambrosoides Against the House Fly, Musca domestica L, Chil Chem Soc., 2010, 55(3), 1-5.

- S.D.G. Aciole, C.F. Piccoli, J.E. Duque, E.V. Costa, M.A. Navarro-Silva, F.A. Marques, B. H. Sales Maia, M.L.B. Pinheiro, M.T. Rebelo, Insecticidal activity of three species of Guatteria (Annonaceae) against Aedes aegypti (Diptera: Culicidae), Rev Colomb Entomol., 2011, 37(2), 262-268.

- R. Pavela, Insecticidal properties of Pimpinella anisum essential oils against the Culex quinquefasciatus and the non-target organism Daphnia magna, Asia-Pac Entomol., 2014, 14, 287-293.

- H. Vatandoost, D.A. Sanei Dehkordi, S.M.T. Sadeghi, B. Davari, F. Karimian, M.R. Abai, M.M. Sedaghat, Identification of chemical constituents and larvicidal activity of Kelussia odoratissima mozaffarian essential oil against two mosquito vectors Anopheles stephensi and Culex pipiens (Diptera: Culicidae), Exp parasitol, 2012, 132(4), 470-474.

- F. Tine-Djebbar, D. Dris, H. Bouabida, N. Soltani, Chemical composition and activity of an Ocimum basilicum essential oil on Culex pipiens larvae: Toxicological, biometrical and biochemical aspects, S Afr J Bot., 2017, 113, 362–369.

- A. Ali, N. Tabanca, B. Demirci, E.K. Blythe, Z. Ali, K.H. Baser, I.A. Khan, Chemical composition and biological activity of four salvia essential oils and individual compounds against two species of mosquitoes, J Agric Food Chem., 2015, 63(2), 447-56.

- M. Govindarajan, M. Rajeswary, G. Benelli, Chemical composition, toxicity and non-target effects of Pinus kesiya essential oil: An eco-friendly and novel larvicide against malaria, dengue and lymphatic filariasis mosquito vectors, Ecotoxicol Environ Saf., 2016, 129, 85-90.

- M. França, O. M. Breda, R.S. B. Douglas, A.M. N. Araujo, C.A. Guedes, The sublethal effects of insecticides in insects, 2017. http://dx.doi.org/10.5772/66461.

- B. Naseri, Z. Abedi, A. Abdolmaleki, M. Jafary-Jahed, E. Borzoui, M. Mansouri, Fumigant toxicity and sublethal effects of Artemisia khorassanica and Artemisia sieberi on Sitotroga cerealella (Lepidoptera: Gelechiidae), J Insect Sci., 2017, 17(5), 1-19.




DOI: http://dx.doi.org/10.13171/mjc101020211198kem

Refbacks

  • There are currently no refbacks.


Copyright (c) 2020 Mediterranean Journal of Chemistry