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Effect of thyme and myrtle leaves essential oils on the quality characteristics of cured sausage during storage

Hayet Ben Haj Koubaier, Ahmed Snoussi, Ismahen Essaidi, Soumaya Hchaichi, Nabiha Bouzouita

Abstract


The aim of this work was to study the effect of adding thyme and myrtle essential oils (TEO and MEO) at different concentrations (0.05 and 1%) on the microbiological and sensorial characteristics of cured sausage during storage (21 days) at +4°C. The chemical composition of essential oils, obtained by hydrodistillation, was analyzed using GC and GC-MS. Twenty and twenty three compounds were identified for thyme and myrtle essential oils, respectively. The major constituents were described as carvacrol (81.4%) for TEO and 1,8-cineole (61%), α-pinene (23.7%) for MEO. In its second part, the present study was conducted to evaluate the in vitro antioxidant and antimicrobial activity of both studied EOs. For this purpose, the DPPH scavenging test and disc-diffusion method was used. Results show that’s both essential oils was able to reduce the stable free radical DPPH with an IC50 of 140 (TEO) and 941 mg/mL (MEO). Significant zone of lysis against all the pathogens studied. On comparing the efficiency of both EOs, T. capitatus EO exhibited higher antibacterial activity against the majority of strains and especially against Klebsiella pneumoniae (DIZ=24.2 mm). During storage, samples containing essential oils showed microbiological parameters stability better than those for the control. The sensorial evaluation shows that 0.05% of either essential oil added was best appreciated by the panelists.


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- L. B. Zeng, Z. R. Zhang, Z. H. Luo, J. X. Zhu, Food Chem., 2011, 125, 456.

- S. Burt, Int. J. Food Microbiol., 2004, 94, 223-253

- D. Yadegarinia, L. Gachkar, M.B. Rezaei, M. Taghizadeh, S.A. Astaneh, I. Rasooli, Phytochem., 2006, 67, 1249–1255

- A. Elaissi, K. Hadj Salah, S. Mabrouk, M.L. Khouja, R. Chemli, F. Harzallah-Skhiri, Food Chem., 2011, 129, 1427-1434

- M. Viuda-Martos, Y. Ruiz-Navajas, J. Fernandez-Lopez, J. Perez-Alvarez, Food Control, 2008, 19, 1130-1138.

- H. Zhou, N. Tao, L. Jia, Food Control, 2014, 37, 277-283

- H. Bagheri, M.Y. Bin Abdul Manap, Z. Solati, Talanta, 2014, 121, 220-228

- J. Gomez - Estaca, A.L.d. Lacey, M.E. Lopez –Caballero, M.C. Gomez - Guillen, P. Montero, Food Microbiol., 2010, 27, 889–896.

- B.Prakash, P. Singh, A. Kedia, N.K. Dubey, Food Res. Int., 2012, 49, 201-208

- A. Snoussi, F. Kachouri, M. M. Chaabouni, N. Bouzouita, J. Essent. Oil Res., 2011, 23 (2), 10-14.

- A. Bouzabata, O. Bazzali, C. Cabral, M. J. Goncalves, M. T. Cruz, A. Bighelli, C. Cavaleiro, J. Casanova, L. Salgueiro, F. Tomi, J. Ethnopharmacol., 2013, 149, 613-620.

- E. Pinto, M. J. Goncalves, K. Hrimpeng, J. Pinto, S. Vaz, L.A. Vale-Silva, C. Cavaleiro, L. Salgueiro,. Ind. Crop. Prod., 2013, 51, 93-99

- V. Aleksic, P. Knezevic, Microbiol. Res., 2014, 169, 240-254.

- C.B. Costa, E. Sendra, J. Fernández-López, J.A. Pérez-Ãlvarez, M. Viuda-Martos, Ind. Crop. Prod., 2014, 50,304-311

- T. Delgado, P. Marinero, S. Asensio, C. M. Manzanera, C. Asensio, B. Herrero, J. A. Pereira, E. Ramalhosa, Food Sci. Technol., 2014, 57, 412-418

- I., Valencia, D., Ansonera, I. Astiasarán, Food Chem., 2007, 104, 1087–1096.

- D. Georgantelis, G. Blekas, P. Katikou, I. Ambrosiadis, D. M. Fletouris, Meat Sci., 2007, 75, 256–264.

- M. A. Fellenberg, H. Speisky, Poultry Sci. J., 2007, 62, 53–69.

- E.H.A. Doolaege, E. Vossen, K. Raes, B. De Meulenaer, R., Verhé, H. Paelinck, S. De Smet, Meat Sci., 2012, 90 (4), 925–931

- G. Pottier-Alapetite, Imprimerie officielle de la république tunisienne : Tunis, 1979.

- W. Jenning, T. Shibamoto, Academic Press: New York, 1980.

- R.P. Adams, Academic Press: New York, 1989.

- M.S. Hanamanthagoud, S.B. Kakkalameli, P.M. Naik, P. Nagella, H.R. Seetharamareddy, H.N. Murthy,Food Chem., 2010, 118: 836–839

- J.P. Guiraud, Dunod: Paris, 1998, 70–244

- L. Hedhili, M. Romdhane, M. Abderrabba, H. Planche, I. Cherif, Flavour Frag. J., 2002, 17, 26–28

- S.Bounatirou, S. Smiti, M.G. Miguel, L. Faleiro, M.N. Rejeb, M. Neffati, M.M. Costa, A.C. Figueiredo, J.G. Barroso, L.G. Pedro, Food Chem., 2007, 105, 146–155

- K. Hosni, I. Hassen, H. Chaâbane, M. Jemli, S. Dallali, H. Sebei, H. Casabianca, Ind. Crop. Prod., 2013, 47, 291–299

- B. Jamoussi, M. Romdhane, A. Abderraba, B. Ben Hassine, A. El Gadri,. Flavour Frag. J., 2005, 20, 274-277

- W. A. Wannes, B. Mhamdi, J. Sriti, , M. Ben Jemia, O. Ouchikh, G. Hamdaoui, , M. E. Kchouk, B. Marzouk, Food Chem. Toxicol., 2010, 48,1362–1370.

- G. Ruberto, M. T. Baratta, Food Chem., 2000, 69, 167–174.

- A. Sokmen, M. Gulluce, H. A. Akpulat, D. Daferera, B. Tepe, M. Polissiou, M. Sokmen, F. Sahin, Food Control, 2004,15, 627–634

- M. Marino, C. Bersani, G. Comi, Int. J. Food Microbiol., 2001, 67, 187-195.

- A. Hayouni, I. Chraief, M. Abedrabba, M. Bouix, J. Leveau, H. Mohammed, M. Hamdi, Int. J. Food Microbiol., 2008, 125, 242–251

- A. Sivropoulou, E. Papanikolaou, C. Nikolaou, S. Kokkini, J. Agricul. Food Chem., 1996, 44, 1202–1205.

- R. S. Farag, Z.Y. Daw, F.M. Hewedi, G.S.A. El-Baroty, J. Food Prot. 1989, 52 (9), 665– 667.

- S.G. Deans, K.P. Svoboda, Flavour Fragr. J. 1990, 5, 187–190.

- S. Pattnaik, V.R. Subramanyam, M. Bapaji, C.R. Kole, Microbios, 1997, 89, 39–46.

- A. Ultee, M.H.J. Bennink, R. Moezelaar, Appl. Environ. Microbiol., 2002, 68, 1561–1568

- P. N. Skandamis, G. J. Nychas, J. Appl. Microbiol., 2001, 91, 1011-1022.

- C. Busattaa, R. S. Vidala, A. S. Popiolskia, A. J. Mossia, C. Darivab, M. R. A. Rodriguesc, F.C. Corazza, M.L. Corazza, J. Vladimir Oliveiraa, R.L. Cansian, Food Microbiol., 2008, 25, 207-211.

- A.M. Martín-Sánchez, C. Chaves-López, E. Sendra, E. Sayas, J.Fenández-López, J.A. Pérez-Ãlvarez, Meat Sci., 2011, 89, 35–44

- H.M.H. Mohamed, H.A. Mansour, Food Sci.Technol., 2012, 45, 79-87

- D.D. Jayasena, C. Jo, Trends Food Sci. Technol., 2013, 34, 96-108

- I. Ozogul, A. Polat, Y. Ozogul, E.K. Boga, F. Ozogul, D. Ayas, Int. J. Food Sci. Technol., 2014, 49, 847–853

- M. Viuda-Martos, Y. Ruiz-Navajas, J. Fernández-López, J.A. Pérez-Ãlvarez, Food Control, 2010, 21, 436–443

- M. Viuda-Martos, Y. Ruiz-Navajas, J. Fernández-López, J.A. Pérez-Ãlvarez, Meat Sci., 2010, 85, 568–576

- M. Oussalaha, S. Caillet, L. Saucier, M. Lacroix, Meat Sci., 73 (2), 2006, 236–244

- R. Casquete, M.J. Benito, A. Martin, S. Ruiz-Moyano, E. Aranda, M.G. Cordoba, Food Control, 2012, 24, 191–198

- ICMSF (The International Commission on Microbiological Speciï¬cations for Foods), Blackwell Scientiï¬c Publications: Oxford, 1986, 181–196

- A.J. Cichoski, R.L. Cansian, D. de Oliveira, I. Gaio, A.G. Saggirato, Food Sci. Technol., 2011, 31, 436-442

- M.D. Bors, M. Tofana, R. Suharoschi, A. Rotar, J. Agroalim. Process.Technol., 2013, 19(3), 314-318

- C.F. Bagamboula, M. Uyttendaele, J. Debevere, Food Microbiol., 2004, 21 (1), 33–42

- P. Penalver, Huerta, B., Borge, C., Astorga, R., Romero, R., Perea, A., Acta Pathol. Microbiol. Immunol. Scand., 2005, 113, 1-6.

- M. Uhart, N. Maks, S. Ravishankar, J. Food Saf., 2006, 26 (2), 115–125.

- A.Fabio, A. Corona, E. Forte, P. Quaglio, The new Microbiologica, 2003, 26(1),115-120

- A. Ben Hsouna, N. Hamdi, R. Miladi, S. Abdelkafi, Chem. Biodiv., 2014, 11, 571-580

- R. Capita, S. Lorente-Marigomez, M. Prieto, C. Alonso-Calleja, J. Food Prot., 2006, 69, 1183–1189

- V. Ferreira, J. Barbosa, S. Vendeiro, A. Mota, M.F. Silva, M.J. Monteiro, T. Hogg, Gibbs, P. Teixeira, Meat Sci., 2006, 73, 570–575

- V. Ferreira, J. Barbosa, J. Silva, J. Vendeiro, S. Mota, F. Silva, M. T. Felício, C. Mena, T. Hogg, P. Gibbs, P. Teixeira, Food Microbiol., 2007, 24, 618–623

- M. Hyldgaard, T. Mygind, R. L. Meyer, Frontiers Microbiol., 2012, 3(12), 1-24

- L. N. Barbosa, V. L. M. Rall, A. A. H. Fernandes, P. I. Ushimaru, I. S. Probst, A. Fernandes, Foodborne Pathog and Dis., 2009, 6(6), 725-728.

- A. C. Seydim, G. Sarikus, Food Res. Int., 2006, 39, 639-644.

- N. Solomakos, A. Govaris, P. Koidis, N. Botsoglou, Meat Sci., 2008, 80, 159-166

- E. Chouliara, A. Karatapanis, I. N. Savvaidis, M. G. Kontominas, Food Microbiology, 2007, 24, 607-617.

- A.M. Martin-Sanchez, C. Chaves-Lopez, E. Sendra, E. Sayas, J. Fenandez-Lopez, J. A. Perez-Alvarez, Meat Sci., 2011, 89, 35-44

- S. M. Al-Reza, A. Rahman, J. Lee, S. C. Kang, Food Chem., 2010, 119, 981-986

- M. Marino, C. Bersani, G. Comi, J. Food Prot., 1999, 62(9), 1017-1023.

- S.F. Mexis, E. Chouliara, M.G. Kontominas, Food Microbiol., 2009, 26, 598-605.

- F. Devlieghere, L. Vermerien, J. Debevere, Int. Dairy J., 2004, 14, 273-285

- G. H. Zhou, X. L. Xu, Y. Liu, Meat Sci., 2010, 86, 119-128.

- I. Chouliara, I. Savvaidis, K. Riganakos, M. G. Kontominas, J. Sci. Food Agricul., 2005, 85, 779-784.

- J. Nguefack, O. Tamgue, J. B. L. Dongmo, C. D. Dakole, V. Leth, H. F. Vismer, P. H. Amvam Zollo, A. E. Nkengfack, Food Control, 2012, 23, 377-383.




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