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Phytochemical analysis, antioxidant activity, and nephroprotective effect of the Raphanus sativus aqueous extract

Hassan Zrouri, Amine Elbouzidi, Mohamed Bouhrim, Noureddine Bencheikh, Loubna Kharchoufa, Sabir Ouahhoud, Hayat Ouassou, Soufiane El Assri, Mohammed Choukri

Abstract


In Morocco, Raphanus sativus is a widespread traditional medicinal plant used to treat various kidney diseases such as nephropathy.

Objective: The present study aims to evaluate the protective effect of the R. sativus aqueous extract against the gentamicin-induced acute nephrotoxicity in rats.

Methods: Rats were randomly separated into four groups (n=6; ♂/ ♀=1). The control group was treated only with distilled water (10 mL/kg; p.o). The gentamicin group was treated with distilled water (10 mL/kg; p.o) and injected intraperitoneally by the gentamicin (80 mg/kg; i.p). R. sativus groups were treated with the aqueous extracts of this plant at a dose of (200 or 400 mg/kg; p.o) and injected by the gentamicin (80 mg/kg; i.p). The plasma Creatinine, Urea, Uric Acid, Albumin, Total Protein, Alanine transaminase, Aspartate transaminase, and Calcium levels were measured. The urinary creatinine, urinary Calcium, urinary volume, water intake, creatinine clearance, body weight gain, relative right kidney weight, and kidney malondialdehydes were determined.

Results: This study showed that the daily pretreatment with R. sativus aqueous extract at two doses of 200 and 400 mg/kg, p.o prevented the rats from the gentamicin-induced nephrotoxicity. Moreover, the R. sativus aqueous extract showed a high amount of polyphenols and flavonoids and a significant antioxidant activity.

Conclusion: R. sativus aqueous extract, as a novel natural product, may have preventive properties against gentamicin-induced nephrotoxicity in rats. The present study describes new areas of investigation to introduce better therapeutic agents for renal disorders and dysfunction.


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- M. Salai Bojan, R. Rajappa, D. R. K. Vijayakumar, J. Gopalan, Protective effect of Raphanus sativus on D-galactosamine induced nephrotoxicity in rats, Nutr Clin Metab, 2016, 30, 22–28.

- S. J. Y, S. Nikhat, J. A. Khan, S. Zohaib, Nephro-protective concept and drugs described in Unani system of medicine : A review, 2018, 2, 36–39.

- S. Fatima, A. N. K. Yusufi, R. Mahmood, Effect of cisplatin on renal brush border membrane enzymes and phosphate transport, Hum Exp Toxicol, 2004, 23, 547–554.

- M. Ouédraogo, A. Lamien-Sanou, N. Ramdé, A. S. Ouédraogo, M. Ouédraogo, S. P. Zongo, O. Goumbri, P. Duez, P. I. Guissou, Protective effect of Moringa oleifera leaves against gentamicin-induced nephrotoxicity in rabbits, Exp Toxicol Pathol, 2013, 65, 335–339.

- M. Bouhrim, H. Ouassou, E. Loukili, M. Ramdani, H. Mekhfi, A. Ziyyat, A. Legssyer, M. Aziz, M. Bnouham, Antidiabetic effect of Opuntia dillenii seed oil on streptozotocin-induced diabetic rats, Asian Pac J Trop Biomed, 2019, 9, 381–388.

- W. M. Haschek, C. G. Rousseaux, M. A. Wallig, B. Bolon, R. Ochoa, Haschek and Rousseaux’s handbook of toxicologic pathology, Academic Press, 2013.

- P. Chatterjee, A. Mukherjee, S. Nandy, Protective effects of the aqueous leaf extract of Aloe barbadensis on gentamicin and cisplatin-induced nephrotoxic rats, Asian Pac J Trop Biomed, 2012, 2, S1754–S1763.

- P. M. Tulkens, Nephrotoxicity of aminoglycoside antibiotics, Toxicol Lett, 1989, 46, 107–123.

- H. T. LEW, S. W. FRENCH, Tetracycline nephrotoxicity and nonoliguric acute renal failure, Arch Intern Med, 1966, 118, 123–128.

- B. M. Tune, Nephrotoxicity of beta-lactam antibiotics: mechanisms and strategies for prevention, Pediatr Nephrol, 1997, 11, 768–772.

- J. Nagai, M. Takano, Molecular aspects of renal handling of aminoglycosides and strategies for preventing the nephrotoxicity., Drug Metab Pharmacokinet, 2004, 19, 159–170.

- J. P. Morin, G. Viotte, A. Vandewalle, F. Van Hoof, P. Tulkens, J. P. Fillastre, Gentamicin-induced nephrotoxicity: A cell biology approach, Kidney Int, 1980, 18, 583–590.

- A. G. G. Coutinho, S. M. P. Biscaia, R. Fernandez, A. L. Tararthuch, The aminoglycoside antibiotic gentamicin is able to alter metabolic activity and morphology of MDCK-C11 cells: A cell model of intercalated cells, Brazilian J Med Biol Res, 2018, 51, 1–7.

- F. C. Luft, M. N. Yum, P. D. Walker, S. A. Kleit, Gentamicin gradient patterns and morphological changes in human kidneys, Nephron, 1977, 18, 167–174.

- P. Balakumar, A. Rohilla, A. Thangathirupathi, Gentamicin-induced nephrotoxicity: Do we have a promising therapeutic approach to blunt it?, Pharmacol Res, 2010, 62, 179–186.

- J. M. Lopez-Novoa, Y. Quiros, L. Vicente, A. I. Morales, F. J. Lopez-Hernandez, New insights into the mechanism of aminoglycoside nephrotoxicity: An integrative point of view, Kidney Int, 2011, 79, 33–45.

- C. Yanagida, K. Ito, I. Komiya, T. Horie, Protective effect of fosfomycin on gentamicin-induced lipid peroxidation of rat renal tissue, Chem Biol Interact, 2004, 148, 139–147.

- M. Tavafi, H. Ahmadvand, P. Toolabi, Inhibitory effect of olive leaf extract on gentamicin-induced nephrotoxicity in rats, 2012.

- C. Kang, H. Lee, D. Y. Hah, J. H. Heo, C. H. Kim, E. Kim, J. S. Kim, Protective effects of Houttuynia cordata Thunb. on gentamicin-induced oxidative stress and nephrotoxicity in rats, Toxicol Res, 2013, 29, 61–67.

- R. M. P. Gutiérrez, R. L. Perez, Raphanus sativus (Radish): their chemistry and biology., ScientificWorldJournal, 2004, 4, 811–837.

- A. R. S. Devraj Shetty, Rajesh Kamath, Pratibha Bhat, Karunakar Hegde*, ANTHELMINTIC ACTIVITY OF ROOT EXTRACT OF RAPHA?US SATIVUS, Pharmacologyonline, 2011, 1, 675–679.

- K. Agarwal, R. Varma, Ethnobotanical study of antilithic plants of Bhopal district, J Ethnopharmacol, 2015, 174, 17–24.

- J. P. B. and M. J. Rahul Deo Yadav, S. K. Jain, Shashi Alok, Alok Mahor, Herbal Plants Used in the Treatment of Urolithiasis : a Review, Int J Pharm Res Dev, 2013, 5, 66–70.

- S. S. Beevi, L. N. Mangamoori, B. B. Gowda, Polyphenolics profile and antioxidant properties of Raphanus sativus L., Nat Prod Res, 2012, 26, 557–563.

- M. Ahn, R. K. Koh, G. O. Kim, T. Shin, Aqueous extract of purple Bordeaux radish, Raphanus sativus L. ameliorates ethanol-induced gastric injury in rats, Orient Pharm Exp Med, 2013, 13, 247–252.

- F. R. G. Terras, H. M. E. Schoofs, M. F. C. De Bolle, F. Van Leuven, S. B. Rees, J. Vanderleyden, B. P. A. Cammue, W. F. Broekaert, Analysis of two novel classes of plant antifungal proteins from radish (Raphanus sativus L.) seeds, J Biol Chem, 1992, 267, 15301–15309.

- R. Vargas S., R. M. Perez G., S. Perez G., M. A. Zavala S., C. Perez G., Antiurolithiatic activity of Raphanus sativus aqueous extract on rats, J Ethnopharmacol, 1999, 68, 335–338.

- M. N. Ghayur, A. H. Gilani, Gastrointestinal stimulatory and uterotonic activities of dietary radish leaves extract are mediated through multiple pathways, Phyther Res, 2005, 19, 750–755.

- O. Folin, V. Ciocalteau, Tyrosine and Tryptophane in Proteins, J Biol Chem, 1927, 73, 627–648.

- D.-O. Kim, O. K. Chun, Y. J. Kim, H.-Y. Moon, C. Y. Lee, Quantification of polyphenolics and their antioxidant capacity in fresh plums, J Agric Food Chem, 2003, 51, 6509–6515.

- M. Bouhrim, N. E. Daoudi, H. Ouassou, A. Benoutman, E. H. Loukili, A. Ziyyat, H. Mekhfi, A. Legssyer, M. Aziz, M. Bnouham, Phenolic Content and Antioxidant, Antihyperlipidemic, and Antidiabetogenic Effects of Opuntia dillenii Seed Oil, Sci World J, 2020, 2020.

- O. OECD, Guideline for the Testing of Chemicals. Acute Oral Toxicity e Acute Toxic Class Method: Test No-423, Organ Econ Coop Dev, 2001.

- H. A. A. Twaij, A. Kery, N. K. Al-Khazraji, Some pharmacological, toxicological and phytochemical investigations on Centaurea phyllocephala, J Ethnopharmacol, 1983, 9, 299–314.

- T. M. M. LC, Estimation of LD50 and its error by means of log-probit graph paper, Proc Soc Exp Biol Med, 1944, 57, 261.

- B. T. Doumas, W. A. Watson, H. G. Biggs, Albumin standards and the measurement of serum albumin with bromcresol green, Clin Chim acta, 1971, 31, 87–96.

- C. Bourguignon, A. M. Dupuy, T. Coste, F. Michel, J. P. Cristol, Evaluation of NM-BAPTA method for plasma total calcium measurement on Cobas 8000®, Clin Biochem, 2014, 47, 636–639.

- H. Talke, G. E. Schubert, Enzymatic urea determination in the blood and serum in the Warburg optical test, Klin Wochenschr, 1965, 43, 174.

- R. J. Henry, Clinical chemistry, principles and technics, 1964.

- A. Karmen, F. Wróblewski, J. S. LaDue, Transaminase activity in human blood, J Clin Invest, 1955, 34, 126–133.

- A. G. Gornall, C. J. Bardawill, M. M. David, Determination of serum proteins by means of the biuret reaction, J Biol Chem, 1949, 177, 751–766.

- P. et al Fossati, Use of 3,5-dichloro-2-hydroxybenzenesulfonic acid/4-aminophenazone chromogenic system in direct enzymic assay of uric acid in serum and urine, Clin Chem, 1880, 26, 227–231.

- H. Ohkawa, N. Ohishi, K. Yagi, Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction, Anal Biochem, 1979, 95, 351–358.

- R. Goyeneche, S. Roura, A. Ponce, A. Vega-Gálvez, I. Quispe-Fuentes, E. Uribe, K. Di Scala, Chemical characterization and antioxidant capacity of red radish (Raphanus sativus L.) leaves and roots, J Funct Foods, 2015, 16, 256–264.

- S. S. Beevi, L. N. Mangamoori, B. B. Gowda, Polyphenolics profile and antioxidant properties of Raphanus sativus L., Nat Prod Res, 2012, 26, 557–563.

- K. S. Jamuna, C. K. Ramesh, R. Mahmood, M. Pallavi, A. R. S. J, Effect of different extraction methods on total phenolic content and antioxidant activities of Raphanus sativus, Int J Bioassays, 2015, 4, 4653–4657.

- I. V Berezovskaya, Classification of substances with respect to acute toxicity for parenteral administration, Pharm Chem J, 2003, 37, 139–141.

- P. K. Govindappa, V. Gautam, S. M. Tripathi, Y. P. Sahni, H. L. S. Raghavendra, Effect of Withania somnifera on gentamicin induced renal lesions in rats, Brazilian J Pharmacogn, 2019, 29, 234–240.

- M. Tavafi, Protection of renal tubules against gentamicin induced nephrotoxicity., J Ren Inj Prev, 2013, 2, 5–6.

- D. H. El-Kashef, A. E. El-Kenawi, M. A. Rahim, G. M. Suddek, H. A. Salem, Agmatine improves renal function in gentamicin-induced nephrotoxicity in rats, Can J Physiol Pharmacol, 2016, 94, 278–286.

- A. Erdem, N. Ü. Gündoǧan, A. Usubütün, K. Kilinç, Ş. R. Erdem, A. Kara, A. Bozkurt, The protective effect of taurine against gentamicin-induced acute tubular necrosis in rats, Nephrol Dial Transplant, 2000, 15, 1175–1182.

- S. Kalayarasan, P. N. Prabhu, N. Sriram, R. Manikandan, M. Arumugam, G. Sudhandiran, Diallyl sulfide enhances antioxidants and inhibits inflammation through the activation of Nrf2 against gentamicin-induced nephrotoxicity in Wistar rats, Eur J Pharmacol, 2009, 606, 162–171.

- M. A. Perazella, Pharmacology behind common drug nephrotoxicities, Clin J Am Soc Nephrol, 2018, 13, 1897–1908.

- P. Randjelović, S. Veljković, N. Stojiljković, D. Sokolović, I. Ilić, Gentamicin nephrotoxicity in animals: Current knowledge and future perspectives, EXCLI J, 2017, 16, 388–399.

- S. Ravikumar, M. Gnanadesigan, Hepatoprotective and Antioxidant Properties of Rhizophora mucronata Mangrove Plant in CCl 4 Intoxicated Rats, J Exp Clin Med, 2012, 4, 66–72.

- E. M. Hosaka, O. F. P. dos Santos, A. C. Seguro, M. de F. F. Vattimo, Effect of cyclooxygenase inhibitors on gentamicin-induced nephrotoxicity in rats, Brazilian J Med Biol Res, 2004, 37, 979–985.

- S. A. Abuelezz, N. Hendawy, S. Abdel Gawad, Alleviation of renal mitochondrial dysfunction and apoptosis underlies the protective effect of sitagliptin in gentamicin‐induced nephrotoxicity, J Pharm Pharmacol, 2016, 68, 523–532.

- M. Bouhrim, H. Ouassou, M. Choukri, H. Mekhfi, A. Ziyyat, A. Legssyer, M. Aziz, M. Bnouham, Hepatoprotective effect of Opuntia dillenii seed oil on CCl4 induced acute liver damage in rat, Asian Pac J Trop Biomed, 2018, 8, 254–260.

- M. R. Khan, I. Badar, A. Siddiquah, Prevention of hepatorenal toxicity with Sonchus asper in gentamicin treated rats, BMC Complement Altern Med, 2011, 11, 3–11.

- C. R. Patil, R. B. Jadhav, P. K. Singh, S. Mundada, P. R. Patil, Protective effect of oleanolic acid on gentamicin induced nephrotoxicity in rats, Phyther Res An Int J Devoted to Pharmacol Toxicol Eval Nat Prod Deriv, 2010, 24, 33–37.




DOI: http://dx.doi.org/10.13171/mjc02101211565lk

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