Cover Image

The determination of the folic acid and its selected derivatives

Barbara Krawczyk, Dominik Szczukocki, Justyna Stachniuk, Radosław Dałkowski, Marek Zieliński, Renata Juszczak, Ewa Miękoś, Robert Zakrzewski

Abstract


Folic acid (FA) belongs to vitamins from group B. The most commonly used drug from a group of structural analogues of FA is methotrexate (MTX), showing antiproliferatative properties. Unfortunately, the action of MTX is not selective. Toxic effects of methotrexate may be partially abolished by the use of leucovorin (calcium salt of folinic acid, FNA) in conserving therapy. The aim of the presented paper was to develop a new analytical procedure using the capillary electrophoresis, allowing the separation and the quantitative marking of the folic acid, methotrexate and calcium salt of folinic acid. Optimal conditions for the electrophoretic separation were obtained for the following parameters: buffer - 25 mmol/L Na2B4O7,pH 9,76; voltage -30 kV, measurement temperature -30 °C, the introduction of a sample to the capillary - hydrodynamically 6 s (0,5 psi). These parameters allowed to obtain LOD 0,6 μM for FA, 0,65 μM for FNA and 0,7 μM for MTX.


Full Text:

PDF

References


- Z. Szakács and B. Noszál, Electrophoresis, 2006, 27, 2299- 3409.

- K. Orzechowska-Juzwenko, In Farmakologia. Podstawy farmakopeii: Leki przeciwnowotworowe, ed. by W. Kostowski and Z. Herman, Wydawnictwo Lekarskie PZWL: Warszawa,2008, 400 -444.

- C.Y. Kuo, H.L. Wu, and S.M. Wu, Anal. Chim.Acta, 2002,471, 211-217.

- M.M. Huang, L. Penn, J. Bongers and S. Burman, J. Chromatogr. A, 1998, 828, 303 - 309.

- D.A. El-Hady, N.A. El-Maali, R. Gotti, C. Bertucci, F. Mancini and V.Andrisano, J. Pharm. Biomed. Anal., 2005, 37, 919 – 925.

- L. Fotsing, M. Fillet, I. Bechet, P. Hubert and J.Crommen, J. Pharm. Biomed. Anal., 1997, 15, 1113-1123.

- D.B. Gomi, L.L. González and D.G. Álvarez, Anal. Chim.Acta, 1999, 396, 55 - 60.

- R.H.F. Cheung, P.D. Morrison, D.M. Small andP.J. Marriott, J. Chromatogr. A, 2008, 1213, 93-99.

- R.H.F. Cheung, J.G. Hughes, P.J. Marriott and D.M. Small, Food Chem., 2009, 112, 507-514.

- U.D. Uysal, E.M. Oncu – Kaya and M. Tuncel, Chromatographia, 2010,71, 653 – 658.

- Z.K. Shihabi, J. Chromatogr. A,1998, 807, 27-36.

- K. Michail and M.S. Moneeb, J. Pharm. Biomed. Anal., 2011, 55, 317 – 324.

- Z. Zhu, H. Wu, S. Wu, Z. Huang, Y. Zhu and L. Xi, J. Chromatogr. A, 2013, 1283, 62 -67.

- J. Rodríguez Flores, G. Castañeda Peñalvo, A. Espinosa Mansilla and M.J. Rodríguez Gómez, J. Chromatogr. B, 2005,819, 141-147.

- C.Y. Kuo, H.L. Wu, H.S. Kou, S.S. Chiou, D.C. Wu and S.M. Wu, J. Chromatogr. A, 2003, 1014, 93-101.

- H.L. Cheng, Y.M. Liao, S.S. Chiou, S.M. Wu, Electrophoresis, 2008,29, 3665 – 3673.

- H.L. Cheng, S.S. Chiou, Y.M. Liao, C.Y. Lu, Y.L. Chen and S.M. Wu, Anal. Bioanal. Chem., 2010, 398, 2183-2190.

- Z. Witkiewicz andJ. Kałużna – Czaplińska, Podstawy chromatografii i technik elektromigracyjnych, Wydawnictwo Naukowo – Techniczne: Warszawa,2013.

- J. Arcotand, A.Shrestha, Trends Food Sci. Tech., 2005, 16, 253-266.

- N. Şanli, S. Şanli and G. Alsancak, J. Chem. Eng. Data, 2010, 55, 2695-2699.

- ed. by P. Konieczka and J. Namieśnik Ocena i kontrola jakości wyników pomiarów analitycznych, Wydawnictwo Naukowo – Techniczne: Warszawa, 2007.




DOI: http://dx.doi.org/10.13171/mjc.3.4.2014.09.24.12.29

Refbacks

  • There are currently no refbacks.


Copyright (c) 2015 Mediterranean Journal of Chemistry