Cover Image

Chemical characterization and antibacterial activity of the essential oils of Tetraclinis articulata (Vahl) from Morocco

Hannou Zerkani, Imane Tagnaout, Aziz Dirioiche, Imad Adadi, Jamila El Karkouri, Guy Stéphane Padzys, Touriya Zair

Abstract


The objective of this study was to identify new bioactive substances through the phytochemical study and evaluation of the antibacterial activity of the essential oils of Tetraclinis articulata (Vahl). Harvested in April 2016, in the region of Khenifra (Middle Atlas-Morocco). Separate of hydrodistillation extracts of the three organs (branches, leaves and fruits) of Tetraclinis articulate were performed. The yields of essential oils are 0.92% for fruits; 0.41% for branches and 0.61% for leaves. The GC/MS analysis allowed the identification of 33 compounds in essential oil leaves, 20 compounds in excess fruits and 58 compounds in branches. The main compounds are bornyl acetate (38.54%) and α-pinene (6.71%) for leaves. α-pinene (22.12%); 13-epi-mannol (3.58%) and retinol (3.44%) for branches. Bornyl acetate (19.96%); α-pinene (12.47%) and retinol (11.98%) for fruits. The antibacterial properties of the essential oil of leaves of Tetraclinis articulata (Vahl) were tested in clinical bacterial strain. The essential oils inhibit the growth of Escherichia coli, Staphylococcus aureus and Klebsiella pneumoniae at 1.2 µl/ml; 2.4 µl/ml and 9.7 µl/ml respectively. Indeed, the resistance of bacteria to antibiotics is known for its adverse effects on human health, to escape this problem, it is necessary to exploit the antibiotics from aromatic and medicinal plants.

Full Text:

PDF

References


- P. Boudy, Économie forestière Nord-Africaine : II, Fasc1 : monographie des essences forestières. Paris, Larousse,1950.

- JE. Dakak, La qualité du bois de Thuya de Maghreb (Tetraclinis articulata) et ses conditions de développement sur ses principaux sites phytoécologiques de son bloc méridional au Maroc. Nancy, France, National School of Rural Engineering, Waters and Forests, 2002.

- D.R.E.F. (Direction Régionale des Eaux et Forêts), Thuya : importance écologique et économique, Florae Libycae Prodromus. Genève, Romet, 2002, 330.

- K. Hadjadj and A. Letreuch Belarouci, Bibliographic synthesis about the barbary thuja (Tetraclinis articulata (Vahl) Mast.), Geo-Eco-Trop, 2017, 41, 1, 13-27.

- M. Hachi, T. Hachin, N. Belahbib, J. Dahmani, L. Zidane, Contribution à l'étude floristique et ethnobotanique de la flore médicinale utilisée au niveau de la ville de Khénifra (Maroc), International Journal of Innovation and Applied Studies, 2015, 11, 754-770.

- H. Zerkani, I. Tagnaout, T. Zair, Ethnobotanical survey and inventory of medicinal flora in the rural municipalities of Ait Ishaq, Tighassaline, El-Hammam and Ageulmam azegza-Khenifra province, Morocco, Journal of Chemical and Pharmaceutical Research, 2015, 7(8), 611-627.

- A. Ziyyat, A. Legssyer, H. Mekhfi, A. Dassouli, M. Serhrouchni, W. Benjelloun, Phytotherapy of hypertension and diabetes in Oriental Morocco. Journal of Ethnopharmacology, 1997, 58, 45-54.

- J. Bellakhdar, La pharmacopée marocaine traditionnelle. Médecine arabe ancienne et savoirs populaires, Paris - Rabat, Ibis Press - Eds Le Fennec,1997, 764.

- M. Bourkhiss, M. Hnach, B. Bourkhiss, M. Ouhssine, A. Chaouch, Composition chimique et propriétés antimicrobiennes de l'huile essentielle extraite des feuilles de Tetraclinisarticulata (Vahl) du Maroc. Afrique Science, 2007, 03(2), 232-242.

- J.F. Clevenger, Apparatus for the determination of volatile oil. J. Am. Pharm. Assoc, 1928, 17(4), 346-351.

- Afnor, Huile essentielle, recueil des normes françaises. 5th edition, échantillonnage et méthodes d'analyse, 2 spécifications, AFNOR, Paris ,1996.

- E. Kováts, Gas chromatographic characterization of organic substances in the retention index system. Advances in Chromatography, 1965, 1, 229-247.

- R.P. Adams, Identification of Essential Oils Components by Gas Chromatography/Mass Spectroscopy,Allured, Carol Stream, IL. 4th edition, 2007, 804.

- F.J. Pérez, J. Ormeño-Nuñez, Root exudates of wild oats: Allelopathic effect on spring wheat. Phytochemistry 1991, 30:2199-2202.

- E.H. Lennette, J.P. Truant, C.H. Spaulding, C. Abella, Manual de microbiología clínica, Salvat Editores,1981.

- F.B. Toumi, M. Benyahia, L. Hamel, H. Mohamedi, L. Boudaghen, étude comparative de la composition chimique des huiles essentielles de Tetraclinis articulata (Vahl) Masters originaire d'Algérie. Acta Bot. Gallica, 2010, 158 (1), 93-100.

- B. Bourkhiss, M. Ouhssine, M. Hnach, M. Bourkhiss, B. Satrani and A. Farah, Composition chimique et bioactivié de l’huile essentielle des rameaux de Tetraclinis articulata,Bull. Soc. Pharm. Bordeaux, 2007, 146, 75-84.

- AF.Barrero, MM.Herrador, P.Arteaga, J.Quilez, M.Akssira, F.Mellouki, S.Akkad ,Chemical composition of the essential oïl of leaves and wood of Tetraclinis articulata(Vahl) Masters, J .Ess. Oil. Res, 2005, 17, 166-168.

- B. Satrani , A. Farah , M. Talbi , Effet de la distillation sur la composition chimique et l'activité antimicrobienne des huiles essentielles de Myrte (Myrtuscommunis L.) du Maroc. Acta Bot. Gallica, 2006, 153(2), 235-242.

- O. Tzakou, E. Verykokidou, V. Roussis,

L. Chinou, Chemical Composition and Antibacterial Properties of Thymus longicaulis subsp. chaoubardii Oils: Three Chemotypes in the Same Population, Journal of Essential Oil Research, 1998, (10), 97-99.

- S.O.S. Hassane, M. Ghanmi, B. Satrani, et al., Composition chimique et bioactivité des huiles essentielles de deux provenances d’Ocimum canum S. de l’île de la Grande Comore. Phytotherapie,2011,9,18-24.




DOI: http://dx.doi.org/10.13171/mjc851907076hz

Refbacks

  • There are currently no refbacks.


Copyright (c) 2019 Mediterranean Journal of Chemistry