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The influence of feeding laying hens N-3 and N-6 fatty acids sources on blood antioxidants status: A comparative study

Sara Ahmed Mohamed, Mohamed Elshiekh Barri, Salwa Mohamed elbasheir, Samia Husien Abdlrahman, Ali Shamat, Hala Elrayah Ahmad, Safa Berair Yousif

Abstract


The objective of this study was to determine the effects of dietary supplementation with different fat sources on blood oxidative status parameters. Seventy five (Hisex) bird, 20 week old, were obtained from Animal Production Research Center (kuku), divided into three groups (n=25) for each one. The control group (A) was under control diet based on corn, maintains the (NRC, 1994) requirements for laying hens, the experimental groups, supplemented by 10% flax seeds (B), and 10% sunflower seeds (C), added to the diet. The trial continued for eight weeks, blood samples were collected once per month (week 4 and 8), in EDTA coated vials, immediately placed into iced-container, centrifuged at 3000rpm/20 min. Samples were separated in aliquot, and stored at -20ºC and -80ºC until analysis.

Plasma analysis for enzymatic and non-enzymatic antioxidants, revealed no significant different level of uric acid, malondialdehyde (MDA) and vitamin C, between the control group and the treated groups, while catalase (CAT), superoxide dismutase (SOD), and vitamin A, levels were significantly enhanced by the addition of 10% flax seeds and 10% sunflower seeds. Vitamin E concentration was significantly elevated in group (C), compared to the control group (A) and group (B).


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Durackova Z. (2010). Some current insights into oxidative stress. Physiol. Res; 59: 459-469.

Eboh Abraham Sisein. (2014). Biochemistry of Free Radicals and Antioxidants, Scholars Academic Journal of Biosciences (SAJB) ISSN 2321-6883 (Online) Sch. Acad. J. Biosci, 2(2): 110-118.

Halliwell B. (1996). Uric acid: an example of antioxidant evaluation. In: Cadenas E, Packer L. editors. Handbook of antioxidants. New York: Marcel Dekker’s 243-56.

Rahman K. (2003). Garlic and aging: new insights into an old remedy. Ageing Res Rev. (2):39-56.

Eboh Abraham Sisein. (2014). Biochemistry of Free Radicals and Antioxidants, Scholars Academic Journal of Biosciences (SAJB) ISSN 2321-6883 (Online) Sch. Acad. J. Biosci; 2(2): 110-118.

Lin H., Decuypere E., Buyse J. (2006). Acute heat stress induces oxidative stress in broiler chickens. Comp. Biochem. Physiol. A Mol. Integr. Physiol, (144): 11-17.

Liu H.N., Liu Y., Hu L.L., Suo Y.L., Zhang L., Jin F., Feng X.A., Teng N., L i Y. (2014). Effects of dietary supplementation of quercetin on performance, egg quality, cecal microflora populations, and antioxidant status in laying hens. Poultry Sci., (93): 347-353.

Safary H., Daneshyar M. (2012). Effect of dietary sodium nitrate consumption on egg production, egg quality characteristics and some blood indices in native hens of west Azarbaijan province. Asian-Aust. J. Anim. Sci, (25): 1611-1616.

Seven I., Aksu T., Seven P.T. (2010). The effects of propolis on biochemical parameters and activity of antioxidant enzymes in broilers exposed to lead-induced oxidative stress. Asian-Aust. J. Anim. Sci, (23): 1482-1489.

Mujahid A., Akiba Y., Toyo M. (2007). Acute heat stress induces oxidative stress and decreases adaptation in young White Leghorn cockerels by down regulation of avian uncoupling protein. Poultry Sci, (86): 364-371.

Ward, O. P.; Singh, A. (2005), Omega-3/6 fatty acids: Alternative sources of production, Proc. Biochem, (40): 3627-3652.

Guedes, A.C.; Amaro, H.M.; Malcata, F. X. (2011), Microalgae as sources of high added-value compounds--a brief review of recent work. Biotechnol. Progr, (27): 597-613.

Huang, C. B.; Ebersole, J. L. (2010). A novel bioactivity of omega-3 polyunsaturated fatty acids and their ester derivatives. Mol. Oral Microbiol, (25):75-80.

Pulz, O.; Gross, W. (2004). Valuable products from biotechnology of microalgae. Appl. Microbiol. Biotechnol, (65): 635-648.

Schmitz, G.; Ecker. (2008).The opposing effects of n-3 and n-6 fatty acids J. Prog. Lipid Res, (47):147-155.

Plaza, M.; Herrero, M.; Cifuentes, A.; Ibanez, E. (2009). Inovative natural functional ingredients from Microalgae, J. Agric. Food Chem, (57): 7159-7170.

Mozaffarian, D.; Wu, J. H. (2011). Omega-3 fatty acids and cardiovascular disease: effects on risk factors, molecular pathways, and clinical events, J. Am. Coll. Cardiol., (58):2047-2067.

Field, C.J.; Schley, P. D. (2004). Evidence for potential mechanisms for the effect of conjugated linoleic acid on tumor metabolism and immune function: lessons from n-3 fatty acids. Am. J. Clin. Nutr, (79):1190-1198.

Das, M.; Zuniga, E.; Ojima, I. (2009). Novel Taxoid-Based Tumor-Targeting Drug Conjugates. Chim. Oggi, (27): 54-56.

Candela, C. G.; Lopez, B. L. M.; Kohen. (2011). Importance of a balanced omega 6/omega 3 ratio for the maintenance of health: Nutritional recommendations, V. L. Nutr. Hosp, (26): 323-329.

Magana, M. M.; Lin S. S.; Dooley K. A.; Osborne T. F. (1997). Sterol regulation of acetyl coenzyme A carboxylase promoter requires two interdependent binding sites for sterol regulatory element binding proteins.,J. Lipid Res, (38): 1630-1638.

Katarzyna Ogniki, Anna Czechi, Iwona Sembratowiczi, Monika Laszewska. (2012). Influence of linseed oil on selected parameters of bloodand production performance of turkey hens, Annales universities Mariae Curie-Sklodowska Lublin – POLONIA, (4):76-83.

Ebeid T. , fayoud a., abou el-soud s., eid y., el-habbak m. (2011). The effect of omega-3 enriched meat production on lipid peroxidation, antioxidative status, immune response and tibia bone characteristics in Japanese quail, Czech Journal of Animal Science, 56(7): 314-324.

Tülay Büyükoğlu, Fatma Karakas Oguz,Mustafa Numan Oguz. (2014). Effects of dietary chicken grill oil and sunflower seed oil on blood and liver oxidant/antioxidant status and liver function tests in laying Japanese quails (Coturnix coturnix japonica), Türk Biyokimya Dergisi Turkish Journal of Biochemistry–Turk J Biochem, 39(4):422-429.

Sammour, S. H. (1999). Proteins of linseed (Linum usitatissimum), extraction and characterization by eletrophoresis. Botanical Academica Sinia, (40):121-126.

Marques, (2011). Effect of flaxseed (Linum usitatissimum L.) prepared by different methods on the biological response of rats. Revista de Nutrição, (24): 131-141.

Vijaimohan, K. Sabitha,M.; Subramaniyam, K.; Anandhan, S; Shyamala,C. and Devi, C.S (2006): Beneficial effects of alpha linolenic acid rich flaxseed oil on growth performance and hepatic cholesterol metabolism in high fat diet fed rats. Life Science, (9). 448-454.

Stodolnik, L. (2005). Rancidity inhibition study in frozen whole mackerel (Scomber scombrus) following flaxseed (Linum usitatissimum) extract treatment. Grasasy Aceites, (56), 198-204.

Ceccarinia L, Macchia M, Flamini G, Cioni PL, Caponi C, Morelli I. (2004). Essential oil composition of Helianthus annuus L. leaves and heads of two cultivated hybrids “Carlos†and “Florom 350â€. Industrial Crops and Products, (19): 13-17.

Verma S, Singh SP, Current and future status of herbal medicines. Veterinary World. (2008), (11): 347-350.

Francisco AM, Ascensión T, José MG, Molinllo, Rosa MV, Diego C. (1996). Potential allelopathic sesquiterpene lactones from sunflower leaves. Phytochemistry; Ecological biochemistry, (43): 1205-1215.

Bohm, Bruce A, Stuessy TF. Flavanoids of the Sunflower Family (Asteraceae). 1st edition. Austria: Springer; 2001.

Javed K. (2001). Quantification of alkaloids, phenols and flavonoids in sunflower (Helianthus annuus L.). African Journal of Biotechnology, (16): 3149-3151.

Ukaya M, Akihisa T, Yasukawa K, Koike K, Takahashi A, Suzuki T, Kimura Y.( 2007), Triterpene glycosides from the flower petals of sunflower (Helianthus annuus) and their anti-inflammatory activity. Journal of Natural Products; (70): 813-816.

Chirva V, Chirva Ya, Cheban PL, Lazur`evskii GV. (1968).The Saponins of Sunflower. Chemistry of Natural Compounds, 42: 140.

Catherine NK, Imungi J K, Okoth M, Momanyi C, Biesalski HK, Vadivel V. (2011). Antioxidant and Antidiabetic Properties of Condensed Tannins in Acetonic Extract of Selected Raw and Processed Indigenous Food Ingredients from Kenya, Journal of Food Science, (76): 560-567.

Kenneth LM, Cecil Randolph S, Iuan A. (1970). Phenolic and sugar components of Armavireo variety sunflower (Helianthus annuus) seed meal. Journal of Agricultural Food Chemistry, (18): 27-32.

National Research Council (NRC), (1994). Nutrient requirements of poultry, Ninth revised edition.

Folch, J., Lees, M and Sloane-Stanely, G. H. (1957). A simple method for the isolation and purification of total lipids from animal tissues. J. Biol. Chem, (226):497-507.

Wang, Y., Sunwoo, H., Cherian, G., and Sim, J.S. (2000). Fatty acid determination in chicken egg yolk: a comparison of different methods. Poultry Science, (79): 1168-1171.

Bablock W. (1998), general regretion procedure for method transformation, J Clin Chem Clin Biochem., (26): 783-790.

Colombo J. P. (1994).Klinisch-chemische Urindiagnostik. Rotkreuz: labovolive- verlagsgesellschaft: 180.

Omaye S.T., Turnbull J.D., Sauberlich H.E., (1979). Selected methods for the determination of ascorbic acid in animal cells, tissues, and fluids. Meth. Enzymol. 62, 3.

Palan PR, Mikhail BS, Basin J, Romney SL. (1973) Plasma Levels of antioxidant beta-carotene and tocopherol in uterine cervix, J. Nutr Cancer, (15): 13-20.

Rutkowski M., Grzegorczyk K., Gendek E., Kedziora J., (2006). Laboratory convenient modification of Bessey method for vitamin A determination in blood plasma J. Physiol. Pharm. 57 (Suppl. 2), 221.

Stara, A., Machova, J. and Velisek, J. (2012). Effect of chronic exposure to simazine on oxidative stress and antioxidant response in common carp (Cyprinus carpio L.). Environmental Toxicology and Pharmacology, 33(2):334-343.

Jin, Y. X., Zhang, X. X., Shu, L. J., Chen, L. F., Sun, L. W., Qian, H. F., Liu, W. P. and Fu, Z. W. (2010). Oxidative stress response and gene expression with atrazine exposure in adult female zebrafish (Danio rerio). Chemosphere, (78):846-852.

İlknur Meriç, (2013), Evaluation of sunflower seed meal in feeds for carp: Antinutritional effects on antioxidant defense system, Journal of Food, Agriculture & Environment, 11 (2): 1128-1132.

Trenzado, C., A. (2006). Antioxidant enzymes and lipid peroxidation in sturgeon Acipenser naccarii and trout Oncorhynchus mykiss. A comparative study. Aquaculture, (254): 758-767.

Bastrop, R., Spangenberg, R. and Jurss, K. 1991. Biochemical adaptation of juvenile carp (Cyprinus carpio L.) to food deprivation. Comparative Biochemistry and Physiology, (98):143-149.

McCord, J. M. and Fridovich, J. (1969). Superoxide dismutase: an enzymatic function for erythrocuprein (hemocuprein). Journal of Biology and Chemistry, (244):6049-6055.

Winston, G. W. and DiGiulio, R. T. 1991. Prooxidant and antioxidant mechanism in aquatic organism. Aquatic Toxicology, (24):143-152.

Tejaswi Chavan, Suresh khadke, Shubhangi Harke, Abhijit Ghadge, Manjiri Karandikar, Vtjaya Pandit, Prabhakar Ranjekar, Omkar Kuikarni and Aniket Kuvalekar. (2013),

hepatoprotective effect of poly unsaturated fatty acids against repeated subacute Acetaminophen dosing in rats, International Journal of Pharma and Bio Sciences, 4(2): 286-295.

Liu L.C. (2009). Effect of unsaturated fatty acid and monensin on rumen and blood fatty acid synthesis in sheep. Ha. Erbing, Northeast Agricultural University.

Becker, B. (1993). Towards the physiological function of uric acid. Free Radical Biology

and Medicine, (14): 615-631.

Chen LY, Zhu WH, Chen ZW, Dai HL, Ren JJ, Chen JH, Chen LQ, Fang LZ. (2007). Relationship between hyperuricemia and metabolic syndrome. J Zhejiang Univ Sci B,(8):593-598.

Abdullah AR, Hasan HA, Raigangar VL. (2009). Analysis of the Relationship of Leptin, High-Sensitivity C-Reactive Protein, Adiponectin, Insulin, and Uric Acid to Metabolic Syndrome in Lean, Overweight, and Obese Young Females. Metab Syndr Relat Disord, (7):17-22. and Medicine, (14): 615-631.

Clausen JO, Borch-Johnsen K, Ibsen H, Pedersen O. (1998). Analysis of the relationship between fasting serum uric acid and the insulin sensitivity index in a population-based sample of 380 young healthy Caucasians. Eur J Endocrinol, (138):63-69.

Matsuura F, Yamashita S, Nakamura T, Nishida M, Nozaki S, Funahashi T, and Matsuzawa Y. (1998) Effect of visceral fat accumulation on uric acid metabolism in male obese subjects:

visceral fat obesity is linked more closely to overproduction of uric acid than subcutaneous fat obesity. Metabolism, (47):929-9.

Hazim J. Al-Daraji, Ali S. Al-Hassani, H.A. Al-Mashadani, W.K. Al-Hayani and H.A. Mirza. (2010), Effect of Dietary Supplementation with Sources of Omega-3 and Omega-6 Fatty Acids on Certain Blood Characteristics of Laying Quail, International Journal of Poultry Science 9 (7): 689-694.

S hivani Saini and Sunil Sharam, (2013). Antidiabetic effect of helianthus annuuls., seeds ethanolic extract in streptozotocin- nicotinamide induced type 2 diabetes mellitus, International Journal of Pharmacy and Pharmaceutical Sciences, 5(2).

Roy, R.N. and B. Guha, (1958). Species differences in regard to Biosynthesis of ascorbic acid. Nature, (182): 319-320.

Fellenberg, M.A. and H. Speisky (2006). Antioxidants: their effect on broiler oxidative stress and its meat oxidative stability. World Poultry Science Journal, (62):53-70.

Dorrell. D.G. and B.A. Vick. (1997). Properties and processing of oilseed sunflower. In: Sunflower Technology and Production (Schneiter, A.A., and Ed.), Amer. Soc. Agron., Madison, Wisconsin, USA.

USDA National Nutrient Database for Standard Reference. (2102). Basic Report 12036, Seeds, sunflower seed kernels, dried.


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