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Electrochemical behavior of Metronidazole (MNZ) at glassy carbon electrode at different concentrations

Ratnesh Das, Aayushi Chanderiya, Atish Roy, Sushil K. kashaw


A robust and straightforward electrochemical methodology based on electrochemical detection of Metronidazole (MNZ) at glassy carbon electrodes was suggested to determine. MNZ formed well-defined reversible cyclic voltammograms on the glassy carbon electrode in the Britton Robinson buffer solution. Various parameters' effects on voltammetric results were also evaluated. For MNZ determination, a differential pulse voltammetric model was introduced and developed. Excellent recovery results for spiked MNZ in pharmaceutical tablet samples were obtained, with recovery rates ranging from 97.44 to 97.51 percent.

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A.S. Evin, E. Serkan, T. Mustafa, T. Begum, Nano-scale selective and sensitive optical sensor for Metronidazole based on fluorescence quenching: 1H-Phenanthro[9,10-d]imidazolylcalix[4]arene fluorescent probe, Analytica Chimica Acta, 2021, 1162, 338494.

B. Nafiseh, F. Nafiseh, M. Alireza, Enhanced antibacterial activity of uniform and stable chitosan nanoparticles containing Metronidazole against anaerobic bacterium of Bacteroidesfragilis, Colloids and Surfaces B Biointerfaces, 2021, 202, 111691.

E.M. Materon, A. Wong, T.A. Freitas, R.C. Faria, O.N. Jr. Oliveira, A sensitive electrochemical detection of Metronidazole in synthetic serum and urine samples using low-cost screen printed electrodes modified with reduced graphene oxide and C60, Journal of Pharmaceutical Analysis, 2021, 11, 646-652.

H. Sylva, H. Marianna, T. Yauheni, C.B. Karla, P. Erich, S. Holešová, Metronidazole clay nanocomposites: Synthesis, structure and antibacterial efficacy, Materials Today, 2020, 37, 21-27.

A. Waszczykowska, D. Zayro, J. Ochocki, P. Jurowski, Clinical Application and Efficacy of Silver Drug in Ophthalmology: A Literature Review and New Formulation of EYE Drops with Drug Silver (I) Complex of Metronidazole with Improved Dosage Form, Biomedicines, 2021, 9,210.

J. Li, X. Hao, C. Wang, H. Liu, L. Liu, X. He, C.C. Sun, Improving the Solubility, Dissolution, and Bioavailability of Metronidazole via Cocrystallization with Ethyl Gallate, Pharmaceutics, 2021, 13, 546.

E.D. Ralph, Clinical pharmacokinetics of Metronidazole, Clin. Pharmacokinet, 1983, 1, 43-62.

T.Y. Ti, H.S. Lee, Y.M. Khoo, Disposition of intravenous Metronidazole in Asian surgical patients, Antimicrob. Agents Chemother., 1996, 40, 2248-2251.

A.R. Gennaro, Remington’s Pharmaceutical Sciences, Miscellaneous Antiprotozoal Drugs, 19th ed., Mack Publishing Company, Easton, Pennsylvania, 1990, 1330.

Martindale, the Extra Pharmacopeia, 29th ed., the Pharmaceutical Press, London, 1989, 666-672.

R.A. Marques, B. Stafford, N. Flynn, W. Sadee, Determination of Metronidazole and misonidazole and their metabolites in plasma and urine by high-performance liquid chromatography, J. Chromatogr., 1978, 1, 163-166.

Y. Wang, L. Yao, X. Liu, CuCo2O4/N-Doped CNTs loaded with molecularly imprinted polymer for electrochemical sensor: Preparation, characterization and detection of Metronidazole, Biosens Bioelectron, 2019,142, 111483.

Z. Abbaspoor, Z. Rabee, S. Najjar, Efficacy and safety of oral tinidazole and Metronidazole in treatment of bacterial vaginosis: a randomized control trial, Int. J. Pharmacol. Res., 2014, 4, 78–83.

J. Muller, P. Schildknecht, N. Muller, Metabolism of nitro drugs metronidazole and nitazoxanide in Giardia lamblia: characterization of a novel nitroreductase (GlNR2), J. Antimicrob. Chemother., 2013, 68, 1781–1789.

W. Liu, J. Zhang, C. Li, L. Tang, Z. Zhang, M. Yang, A novel composite film derived from cysteic acid and PDDA-functionalized graphene: enhanced sensing material for electrochemical determination of Metronidazole, Talanta, 2013, 104, 204–211.

A.T.E. Vilian, K.S. Ranjith, S.J. Lee, Hierarchical dense Ni−Co layered double hydroxide supported carbon nanofibers for the electrochemical determination of Metronidazole in biological samples, Electrochim. Acta, 2020, 354, 136723.

D. Pang, Y. Liu, H. Song, Trace Ti3+- and N-codoped TiO2 nanotube array anode for significantly enhanced electrocatalytic degradation of tetracycline and Metronidazole, Chem Eng J., 2021,405, 126982.

S. Lu, K. Wu, X. Dang, S. Hu, Electrochemical reduction and voltammetric determination of Metronidazole at a nanomaterial thin film coated glassy carbon electrode, Talanta, 2004, 63,


W. Liu, J. Zhang, C. Li, L. Tang, Z. Zhang, M. Yang, A novel composite film derived from cysteic acid and PDDA-functionalized graphene: Enhanced sensing material for electrochemical determination of Metronidazole, Talanta, 2013, 104, 204-211.

A.A. Ensafi, P. Nasr-Esfahani, B. Rezaei, Metronidazole determination with an extremely sensitive and selective electrochemical sensor based on graphene nanoplatelets and molecularly imprinted polymers on graphene quantum dots, Sensors Actuators B Chem., 2018, 270, 192-199.

A. Salimi, M. Izadi, R. Hallaj, M. Rashidi, Simultaneous determination of ranitidine and Metronidazole at glassy carbon electrode modified with single wall carbon nanotubes, Electroanalysis, 2007,19(16), 1668-1676.

S. Yuan, X. Bo, L. Guo, In-situ insertion of multi-walled carbon nanotubes in the Fe3O4/N/C composite derived from iron-based metal-organic frameworks as a catalyst for effective sensing acetaminophen and Metronidazole, Talanta, 2019, 193, 100-109.

B. Zheng, C. Li, L. Wang, Signal amplification biosensor based on DNA for ultrasensitive electrochemical determination of Metronidazole, RSC Adv., 2016, 6(66), 61207-61213.

Y. Baikeli, X. Mamat, F. He, Electrochemical determination of chloramphenicol and Metronidazole using a glassy carbon electrode modified with iron, nitrogen co-doped nanoporous carbon derived from a metal-organic framework (type Fe/ZIF-8), Ecotoxicol Environ Saf., 2020, 204, 111066.

S.E. Berrabah, A. Benchettara, F. Smaili, S. Tabti, A. Benchettara, Electrodeposition of zinc hydroxide on carbon graphite electrode for electrochemical determination of trace copper in water samples using square wave anodic stripping voltammetry, Materials Chemistry and Physics, 2022, 278(7), 125670.



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