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Secondary metabolites and antioxidant activity of seed extracts from Solanum elaeagnifolium Cav.

Houda Feki, Imed Koubaa, Mohamed Damak

Abstract


The aim of this study was to screen various solvent extracts of seeds of Solanum elaeagnifolium to display the phytochemical composition, the total phenolic content, the total flavonoid content and the antioxidant capacity in order to find possible sources for future novel antioxidants in food and pharmaceutical formulations. Various extracts of seeds of Solanum elaeagnifolium were obtained by maceration. The total phenolic content of the different extracts was determined by Folin-Ciocalteu method, and the total flavonoid content was quantified using a method based on the formation of a flavonoid–aluminium complex .The antioxidant activity was essayed through some in vitro models such as the antioxidant capacity by phosphomolybdenum method, radical scavenging activity using 2,2-diphenyl-1-picryl hydrazyl (DPPH) assay and reducing power assay. The acetone extract showed the highest total phenolics (580.99 ± 20.56 mg gallic acid equivalents/g of extract), and the highest total flavonoids (207.61 ± 2.62 mg quercetin equivalents/g of extract). This extract showed an antioxidant activity higher than that of α-tocopherol. These results suggest the potential of Solanum elaeagnifolium against free-radical-associated oxidative damage.

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DOI: http://dx.doi.org/10.13171/mjc.2.5.2014.05.01.22

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